Fig. 4.

A small LPS glycoform correlates with BSAP-3 sensitivity. a Western immunoblot of whole-cell lysates of WT strains probed the adsorbed antiserum of Fig. 3. The arrow indicates a low MW LPS form only present in sensitive strains. b Overlay assays showing the conversion of a BSAP-3-producing strain to a sensitive strain by deletion of the three unique genes in the BvCL09 LPS region (1764-66) and addition of the two glycosyltransferase genes from BvCL10. c, d, e. Silver strain or western immunblot analyses of c, cell lysates or d, e, purified LPS preparations showing the shift in the low MW LPS form when the two BvCL10 GT genes are added to the BvCL09 Δ1764-66 mutant