FIGURE 7.

Expression of key genes encoding enzymes involved in the biosynthesis of the vitamins thiamine (A) and folic acid (B) in roots and nodules of D. glomerata and C. thyrsiflorus. Gene expression levels in roots (R) and nodules (N) are relative to those of EF-1α. THIC, phosphomethylpyrimidine synthase; THI4, bifunctional enzyme hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase; NUDT1, nudix hydrolase and ADCL, 4-amino deoxychorismate lyase. The median and IQR of at least three biological replicates are shown. Differences between R and N are indicated by p-values after student’s t-test followed by FDR multi comparison correction. Y-axis is given in log₁₀ scale.