Figure 4.

Increased intestinal barrier dysfunction in LPS-challenged LysM^ΔCommd10 mice driven by Ly6C^hi monocytes. Commd10^fl/fl (blue) and LysM^ΔCommd10 (red) mice were i.p. injected with LPS (0.2 mg per mouse of similar weight) and sacrificed 24 or 48 h later. Where indicated, MC-21 was injected 12 h prior to LPS stimulation and every 24 h (red border). (A) Fluorometric assessment of FITC dextran plasma signal at 4 h following its oral administration to mice after 24 h of LPS challenge (n ≥ 6). (B) Graphical summary of qRT-PCR assessment of Claudin 1, 2, and 15 gene expressions at 48 h following LPS challenge (n ≥ 7). (C) Indicated tissues were extracted at 48 h following LPS injection. Colony forming units (CFU) were determined and normalized to tissue mass (n = 4). Data were analyzed by unpaired, two-tailed t-test, comparing each time between Commd10^fl/fl and LysM^ΔCommd10 (red stars) or between LysM^ΔCommd10 mice with and without MC-21 treatment (black stars). Data are presented as mean ± SEM with significance: ^*p < 0.05, ^**p < 0.01. Data in (A–C) represent 2–3 independent experiments.