Figure 7.

COMMD10 is downregulated in mouse Ly6C^hi monocytes and their equivalent human CD14^hi monocytes during intestinal inflammation. (A) Splenic and colonic Ly6C^hi monocytes were sorted from steady state mice (solid) or at day 4 of DSS (diagonal). (B,C) Circulating CD14^hi monocytes were sorted from patients with active IBD (black border) or healthy controls (solid). (B) qRT-PCR gene expression of Commd10 and (C) immunoblot demonstrating COMMD10 protein expression. GAPDH was utilized as control (n ≥ 3). (D,E) Biopsies were obtained from colons and terminal ileums of IBD patients with active disease and healthy controls. (D) qRT-PCR analysis of Commd10 gene expression. (E) Immunoblots demonstrating COMMD10 protein expression in colon (left panel) and ileum (right panel) biopsies obtained from IBD patients vs. healthy controls. GAPDH was utilized as controls (n ≥ 14). Data were analyzed by unpaired, two-tailed t-test, comparing each time between IBD with healthy controls. Results are presented as mean ± SEM with significance: ^*p < 0.05, ^***p < 0.001. Data in (A–C) represent a single experiment.