Skip to main content
. 2018 Nov 14;9:2656. doi: 10.3389/fimmu.2018.02656

Figure 3.

Figure 3

Microbiota dysbiosis modify TNFα mRNA expression but not protein production by alveolar macrophages. (A) Gating strategy to analyze alveolar (MerTK+CD64+SiglecF+CD11c+) and interstitial (MerTK+CD64+SiglecF−CD11c−) macrophages by flow cytometry. The total number of (B) alveolar and (C) interstitial macrophages was quantified by flow-cytometry in lung homogenates from M. tuberculosis-infected ABX vs. non-ABX mice 7 days p.i., Data from 2 independent experiments (n = 4–5 mice/group/experiment) were pooled and the graphs show mean ± SD (B) or median with interquartile range (C). Data were analyzed using the Student's t-test (B) or the Mann-Whitney test (C). (D) Left panel, MerTK+CD64+SiglecF+CD11c+ alveolar macrophages were sorted and the expression of TNFα was measured by RT-qPCR in cells from non-ABX, ABX, or FT mice 7 days p.i., Gene expression represents relative Ct value compared to Hprt Ct value (ΔCt) and the mean of ΔCt values in the control group (ΔΔCt). Right panel, cytometry analysis of the percentage of TNFα-producing MerTK+CD64+SiglecF+CD11c+ alveolar macrophages following in vitro stimulation by LPS. Data from 1–2 independent experiments (n = 4–5 mice/group/experiment) were pooled and the graphs show median with interquartile range of the pooled data and were analyzed using the Mann-Whitney test *p < 0.05.