(A) The expression of LC3, P62, Beclin-1, and Pink1 protein was measured by Western blot analysis in kidneys from rats in group Con (Con), group CI-AKI (CI-AKI), group Rap1 (Rap1), and group Rap2 (Rap2). Descriptions of the groups are in the text. Contrast exposure induced autophagy in TEC cells. Rapamycin significantly up-regulated the expression of LC3-II/I, Beclin-1 and down-regulated the expression of P62. (B) Data show the mean ± S.D. Experiments were repeated three times. *P<0.05 compared with Con; #P<0.05 compared with CI-AKI; &P<0.05 compared with Rap1.