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. 2018 Nov 21;38(6):BSR20180240. doi: 10.1042/BSR20180240

Figure 1. Tamoxifen attenuated EMT and decreased β-catenin expression in peritoneal fibrosis induced by HG PDF.

Figure 1

Peritoneal tissues collected after 30 days of exposure to 4.25% HG PDF with or without Tamoxifen (Tamo). (A) Paraffin sections stained with Masson’s trichrome (blue) or incubated with anti-Vimentin antibody (brown, left panel), and quantitated as the thickness and positive area of peritoneum (right panel). Original magnification: 400×. Bar = 100 μm. (B) Frozen sections were incubated with antibodies against β-catenin (red) and nuclei were stained with Hoechst (blue). Original magnification: 400×. Bar = 100 μm. (C,D) Expression levels of E-cadherin, Vimentin, β-catenin, and Snail examined by Western blotting (left panel) and quantitated by densitometry normalized to β-tubulin (right panel) (mean ± S.D., n=5, ***P<0.001 and **P<0.01 compared with control group, ###P<0.001 compared with PDF group).