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. 2018 Nov 21;38(6):BSR20180240. doi: 10.1042/BSR20180240

Figure 4. Tamoxifen inhibited HG-induced up-regulation and nuclear localization of β-catenin and Snail in mPMCs.

Figure 4

mPMCs exposed to NG, HG, HG plus Tamoxifen (HG + Tamo) and HG plus Tamoxifen plus TWS (HG + Tamo + TWS) for 12 h. (A) mPMCs incubated with antibodies against β-catenin (red) and Snail (green). Nuclei were stained with Hoechst (blue). Bar = 40 μm. (B) Expression levels of p-β-catenin, total β-catenin, and Snail in mPMCs examined by Western blotting (left panel) and quantitated by densitometry normalized to β-tubulin (right panel) (mean ± S.D., n=4, ***P<0.001 compared with NG, ###P<0.001 compared with HG, +P<0.05 and +++P<0.001 compared with HG + Tamo; **P<0.01 compared with NG.).