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. 2018 Nov 21;38(6):BSR20180620. doi: 10.1042/BSR20180620

Figure 5. ANRIL regulated the expression of ABCC1 by inhibition of Let-7a.

Figure 5

(A) The expression of ABCC1 and ABCC5 in CRC tumor tissues. (B) The prediction sequence of ANRIL binding Let-7a and Let-7a binding site of ABCC1. The ANRIL expression was negatively correlated with the Let-7a in the right panel. (C) The expression of Let-7a was decreasing in CRC cell lines compared with FHC. (D) The expression of Let-7a in HCT116 and SW480 cells transfected si-NC, si-ANIRL, pcDNA-Vector and pcDNA-ANRIL. (E) The expression of Let-7a in HCT116 and SW480 cells transfected Let-7a negative control (Let-7a NC), Let-7a inhibitor (Let-7a in) and Let-7a mimics (Let-7a mi). (F) The expression of ABCC1 in HCT116 and SW480 cells transfected Let-7a negative control (Let-7a NC), Let-7a inhibitor (Let-7a in) and Let-7a mimics (Let-7a mi). (G) The luciferase reporter assay was performed in HCT116 cell transfected ANRIL wild-type/mut and Let-7a mimics/negative control, as well as ABCC1 wild-type/mut and Let-7a mimics/negative control. Data represent three independent experiments (mean ± SD of triplicate samples); *P<0.05, **P<0.01, ***P<0.001.