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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: J Immunol. 2018 Oct 24;201(11):3161–3165. doi: 10.4049/jimmunol.1800806

Fig 2. Vagus electrical stimulation of SPM production and reduction of eicosanoids.

Fig 2.

Mouse vagus incubated (20min at 37°C with 5 % CO), electrically stimulated (2.5mA 18V direct DC, 20min), or with 109c.f.u. E. coli (3h at 37°C), bioactive metabolomes identified and quantified as in Fig. 1A) PCA 3D score plot; B) Loading 2D plot shows endogenous LM. 3D, 3-dimensional. 2D, 2-dimensional. C) RvD3, RvD4, RvE1, RvE3, and LXB4 (left) increased, LTB4, PGD2, PGE2, PGF2α, and TxB2 (right) diminished. E) LM-SPM-Network pathways visualized with Cytoscape (3.6.1) with mean value changes between unstimulated and stimulated vagus. Up-regulated LM (red), down-regulated (blue). Black circles, not detected; Grey squares, not monitored. A-E are representative from 3 independent animals. Results are mean±SEM, *p<0.05, one-tailed t test.