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. Author manuscript; available in PMC: 2019 Nov 9.
Published in final edited form as: Dev Dyn. 2018 Nov 9;247(11):1199–1210. doi: 10.1002/dvdy.24679

Figure 7 – Stereotypical peptide domains are required for neil3 function.

Figure 7 –

A. Diagram of neil3 protein highlighting stereotypical peptide domains: NEI domain (blue), RanBP-type zinc finger (yellow), and GRF-type zinc fingers (red). Wild type (top line) and mutated versions are depicted. ΔGRF: both GRF zinc fingers deleted; ΔGRF2: second GRF zinc finger deleted; ΔRBP: RanBP zinc finger deleted; D166N: point mutation resulting in aspartate 166 substitution by asparagine. B,C. RNA encoding neil3 containing the D166N mutation (B) or deletions of RanBP- or GRF-type zinc fingers (C) does not rescue the rax knockdown phenotype to the same extent as wild type neil3. Open/white portion of graph bar: normal eye; gray: microphthalmia; black: anophthalmia. X. laevis embryos were injected with rax antisense morpholino oligonucleotides (ASMO) alone or with mRNA encoding wild type or nutated neil3. RNAs and lissamine-tagged MOs were injected into a dorsal blastomere at the 4-cell stage and embryos were screened for one-sided injection and normal, small, or absent eyes at st 41. Number of embryos for each condition (n) is shown below each column in B and C.