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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Aquat Toxicol. 2018 Sep 29;205:25–35. doi: 10.1016/j.aquatox.2018.09.014

Table 1.

Primers used to amplify and sequence Atlantic sturgeon and shortnose sturgeon AHR1

Forward primers for RT-PCR
STAHR1_23F GAAAGCCAGTGCAGAAA
STAHR1_151F GTTATCTCCAAACTGG
STAHR1_423F TGTCATCCACCAGAGTG
STAHR1_592F CTTCCTCCAGAGAATTC
STAHR1_1189F GCTACTGGGGAGGCTGT
STAHR1_1595F TTCAGCAAGATGAGGAG
STAHR1_1852F CAGCAGCAGCTCTGCCA
STAHR1_2146F GAATTGGAGGATTTCCT
STAHR1_2254F GTGTCTATGTACCAGTG
STAHR1_354F GGTTAGTGCCGATGGTTCAG
STAHR1_484F GCGTCAGCTTCACTGGG
STAHR1_−48F CGAGACGGGATGATGAAC
STAHR1_−99F CTGGCTTTTTAAATATAA
STAHR1_−88F AATATAAAAAGCGTCTTC
Reverse Primers for RT-PCR
STAHR1_476R AATTCTGCTCTGTCTTC
STAHR1_608R GAATTCTCTGGAGGAAG
STAHR1_885R TTTTCCTTTGGCATCAC
STAHR1_1205R ACAGCCTCCCCAGTAGC
STAHR1_1611R CTCCTCATCTTGCTGAA
STAHR1_1612R ACTCTTCATCTTGCTGA
STAHR1_1868R TGGCAGAGCTGCTGCTG
STAHR1_2165R TCCAGGAAATCCTCCAA
STAHR1_2383R TATCCATTTTGGAACTT
STAHR1_2502R TGGAAAGCCACTGGATG
STAHR1_2568R CTTCAGAAGGCAGCACTC
STAHR1_2656R CTGCACAGTATAACCAGC
STAHR1_219R GAAGCTTTTGGCTCTCAG
STAHR11268R CCTTTGGCCTTGGCATGC
STAHR11311R CTCTTGATCTCTTGCTC
Primers used for Real Time PCR
STRT_2213F GCCCTCAGGATAGTATGATTACTTCAC
STSNRT_2284R CCGGCAGACACTGGTACATAG
Sturact60F CATTGTCACCAACTGGGATGAC
Sturact125R ACACGCAGCTCATTGTAGAAGGT