Skip to main content
. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: J Immunol. 2018 Oct 19;201(11):3421–3430. doi: 10.4049/jimmunol.1800337

Figure 2. B cell-intrinsic TRAF2 but not TRAF3 deficiency impairs CD40-induced CSR ex vivo.

Figure 2.

(A) Representative FACS data of IgG1 and IgE CSR. Purified B cells from various genotypes were stimulated with anti-CD40/IL-4 or LPS/IL-4 for 4 days and analyzed by flow cytometry. (B) Quantification of FACS data. The percentage of IgG1+ or IgE+ isotype-switched B cells was calculated from triplicates of each indicated genotype in one experiment. LMC: littermate control (TRAF2fl/fl or TRAF3fl/fl); 2KO: B-TRAF2-KO; 3KO: B-TRAF3-KO. Statistical analysis was performed as described in Figure 1. For panel A and B, all experiments were repeated five times independently and representative data from one experiment were shown. (C) Representative FACS data of purified MZ and FO B cells. FO B cells are CD23highCD21low, whereas MZ B cells are CD21highCD23low. (D) Representative FACS data of IgG1 CSR of FO and MZ B cells. Purified FO vs. MZ B cells from B-TRAF2-KO or B-TRAF3-KO mice were stimulated with anti-CD40/IL-4 or LPS/IL-4 for 4 days. For panel C and D, all experiments were repeated three times independently and representative data from one experiment are shown.