Figure 1. LXB₄ enhances memory B cell IgG production.

(A) Purified CD19+ B cells were pretreated with 10 or 100 nM LXB₄, followed by stimulation with different polyclonal activators as shown (MBCC, memory B cell-inducing cocktail). IgG was measured in the supernatant by ELISA after 7–8 days. (B) Purified B cells were treated with LXB₄ alone (50nM) or CpG + anti-IgM as a positive control, for 7 days, and IgG was measured in the supernatant. (C–F) PBMCs or purified CD19+ B cells were stimulated with the memory B cell-inducing cocktail and treated with the indicated concentration of LXB₄. IgG, IgM or IgE levels were measured in the culture supernatants after 7–8 days by ELISA. For (A) and (B), results shown are mean±S.D. for triplicate wells from one representative donor of 3 donors tested. For (C-F), each symbol represents an individual donor and represents the mean of triplicate or quadruplicate cultures. Data analyzed by repeated measures one-way ANOVA with Tukey’s posttest, *p≤0.05, **p≤0.01, ***p≤0.001.