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. 2018 Sep 4;34(6):1105–1110. doi: 10.1007/s12264-018-0281-z

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Regulation of sleep, courtship, and feeding behaviors by P1 neurons. A–D Mild activation of P1 neurons driving dTrpA1 at 27°C using five independent P1 drivers (P1^a–P1^e) inhibited sleep (A, B), but did not affect courtship (C) or feeding (D) [sleep test at 27°C (A, B), n = 32 each; wing-extension test (C), n = 48 each; feeding test (D), n = 10, 10, 10, 9, 9, 10, and 10 (10 flies for each replicate)]. E–H Stronger activation of P1 neurons at 30°C using all P1 drivers affected sleep (E, F), while four drivers (P1^a, P1^c, P1^d, and P1^e) affected courtship (G), and only one (P1^e) affected feeding (H) [sleep test at 30°C (E, F), n = 31, 32, 32, 32, 58, and 32; wing-extension test (G), n = 48 each; feeding test (H), n = 10 each]. ***P < 0.001, one-way ANOVA. N.S., no significant difference. Error bars indicate SEM.