Figure 5.

Y. frederiksenii ΔyacT exhibits attenuated virulence. (A) Y. frederiksenii/pACYC184, Y. frederiksenii ΔyacT, Y. frederiksenii ΔyacT/pACYC184, and Y. frederiksenii ΔyacT /pACYC-yacT were used to infect G. mellonella larvae that were incubated for 10 days at 15°C. Infection doses and TD₅₀ values are indicated in Table 3. In all experiments depicted in the Kaplan–Meier plot, three independent infection experiments per strain were monitored, with groups composed of 15 larvae each. (B) Additionally, larvae infected in parallel were homogenized at the indicated time points and the number of viable Y. frederiksenii cells were enumerated. Gray boxes: Y. frederiksenii, black boxes: Y. frederiksenii ΔyacT. Standard deviations of three replicates are shown.