FIG 1.

Genomic structure of Dectin-1, isoforms resulting from alternative splicing, and influence of the rs2078178 genotype. (A) The structures of CLEC7A and the different isoforms resulting from alternative splicing are shown together with the positions of the polymorphisms. The black lightning bolts indicate the positions of the stop codons introduced through frameshifts generated by alternative splicing. The diagram has been constructed based on the sequence under GenBank accession number NG_016291.1 and data reported previously by Willment et al. (12). (B) Monocytes from buffy coats from healthy donors of the different rs2078178 genotypes were isolated and cultured for mRNA collection. The different isoforms of Dectin-1 were amplified by PCR and separated by electrophoresis on an agarose gel. Abbreviations: Cyto, cytoplasmic tail; TM, transmembrane region; CRD, carbohydrate recognition domain.