FIG 3.

C. perfringens localization in the intestine. (A) PAS and Gram staining of the small intestine of a mouse 1 day after challenge with the F4969 wild-type strain (left) or TH broth alone (right). Numerous Gram-positive rods are visible within the mucus layer. (B) C. perfringens immunohistochemistry on the small intestine of a mouse challenged with the F4969 wild-type strain. Most of the immunopositive staining is present within the mucus layer. Note that no immunostaining was present in the absence of F4969 challenge. TH only indicates challenge with sterile medium (no infection). Magnifications, ×600.