FIG 5.

Microscopy comparison of wild-type and nanI mutant strain abundance in mouse intestine. Note that inactivation of the nanI sialidase gene decreases the amount of C. perfringens F4969 in the intestine of mice. Representative C. perfringens IHC images of the intestines of mice on day 4 postchallenge are shown. Consistent with the findings presented in Fig. 2, lower numbers of the nanI mutant than the wild-type strain were visualized on day 4 in the small intestine, cecum, and colon after bacterial challenge in clindamycin-pretreated mice.