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Primers used in this study
| Primer name | Sequence (5′–3′) | Target gene | Product size (bp) |
|---|---|---|---|
| nanHKOF | AATTGGATGGCTAGGTGGAGTT | nanH | 285 |
| nanHKOR | CAGGTGCTTCCTAAATCGTGAG | ||
| nanIKOF | CAAGAGTTGGTTTTGAGC | nanI | 467 (wild type), 1,367 (null mutant) |
| nanIKOR | AAATAAGGCTGGTATTCTG | ||
| nanJKOF | CTGCAATTCAAGGTGTTGGTG | nanJ | 306 |
| nanJKOR | CTTGTCTTCTAAGCTCATATCC | ||
| CPEnteroF | GGAGATGGTTGGATATTAGG | cpe | 233 |
| CPEnteroR | GGACCAGCAGTTGTAGATA |
