FIG 4.

C. albicans Δ/Δras1 suppressed ROS production. (A) Human neutrophil ROS production upon coincubation with C. albicans WT, Δ/Δras1, Δ/Δefg1, RAS1OE, and ras1/RAS1 complemented strains was detected with luminol chemiluminescence over 150 min at 37°C. As controls, neutrophils were left untreated (negative) or treated with PMA (positive). The graph is a representation of one biological replicate. (B) C. albicans WT, Δ/Δras1, Δ/Δefg1, RAS1OE, and ras1/RAS1 complemented strain ROS production was detected using luminol as indicated for panel A. (C) Total ROS production was obtained by calculating the area under the curve of four independent experiments performed in duplicate. Shown are the means ± SDs from four independent experiments performed in duplicate. Significance was calculated using one-way ANOVA with post hoc Dunnett’s multiple-comparison test. *, P < 0.05.