FIG 1.

Glucose content in zebrafish intestine after 12 h of 1% glucose feeding. One percent glucose was dissolved in autoclaved fish tank water. Five fish were kept in the water for 6, 12, and 24 h of glucose feeding. Fish intestines were homogenized, heated with Benedict’s solution in boiling water, and observed for color formation. Samples were 1× PBS (tube 1), 1% glucose (tube 2), control fish intestine without glucose feeding (tube 3), and fish intestine with 6 h (tube 4), 12 h (tube 5), and 24 h (tube 6) of glucose feeding. The results shown are representative of multiple experiments conducted. The content of glucose in fish intestine is listed in Table 1.