Figure 2.

Effect of conditioned media (CM) produced by pancreatic ductal adenocarcinoma cells on THP‐1 monocytes differentiation. A, Representative images of cells stimulated by corresponding CM for 48 h. Magnification: 100×; Scale bars: 100 μm. B, THP‐1 monocytes were treated with 50% v/v PANC‐1‐CM or BxPC‐3‐CM for 48 h to promote cell differentiation before immunofluorescent staining. Immunofluorescence detection of CD68 (green) and CD163 (red) demonstrated their coexpression in tumor‐driven like macrophages. Magnification: 400×; Scale bars: 20 μm. C, The expression of CD68 and CD163 in different CM‐treated THP‐1 cells was detected by Western blotting. D, The mRNA expression of CD68, CD163, CD204, and HLA‐DR was examined by qRT‐PCR. *P < 0.05