Figure 1.

The colony‐forming ability of CRC cells is suppressed by ginsenoside Rb2. (A) The chemical structure of ginsenoside Rb2. (B and C) Both CRC cell lines HT29 and SW620 were seeded in 96‐well plate and treated with ginsenoside Rb2 with different concentrations. The MTT assay was used for measuring the cell proliferation after 72 h. (D‐F) HT29 and SW620 cells were seeded and treated with ginsenoside Rb2 for 48 h. The apoptotic effect of ginsenoside Rb2 was analyzed using Annexin V assay. EA and LA stand for early apoptosis and late apoptosis, respectively. A representative image was shown (D), and the data were presented as the mean ±SEM of two independent experiments (E and F). (G‐J) Both CRC cell lines HT29 and SW620 were seeded in complete media containing 0.3% of agarose with either DMSO or ginsenoside Rb2 at different concentrations. The number of colony was counted after incubating 15 d. (D and E) A representative image was shown. The statistical analysis is shown (*, P < 0.05; **, P < 0.01). The data are presented as the mean ±SEM of three independent experiments