Figure 3.

Suppressing USP14 expression sensitized GC cells to cisplatin in vitro. A, B, USP14 has high expression at both mRNA and protein level in the indicated six GC cell lines. C, D, A pool of three siRNAs (100 nmol/L in total) were introduced into MKN45 (C) and KATO III (D) cells, respectively. The USP14 expressions in these cells were measured via RT‐qPCR and western blotting. Data, **P < 0.01, ****P < 0.0001. E, F, USP14 deletion reduced cisplatin IC50 of MKN45 cells from 39.2 ± 5.17 to 9.17 ± 2.68 μmol/L (E). Likewise, cisplatin IC50 decreased from 52.24 ± 8.67 μmol/L to 10.56 ± 5.87 in KATO III cells (F)