Figure 5.

USP14 depletion induced apoptosis in cisplatin‐treated GC cells. A, WST‐1 assay were performed to evaluate the cell viability at 24, 48, and 72 h. Knockdown of USP14 had barely any effect on cell viability in MKN45 and KATO III cells. B, Flow cytometry results showed that the proliferation rate was not affected in USP14 deficient cells. C, Knockdown of USP14 did not affect PCNA expression in MKN45 and KATO III cells. D, Flow cytometry showed that reduced USP14 expression increased the apoptotic population of cisplatin‐treated MKN45 cells by 18.2% or KATO III cells by 8.3%, respectively. E, Depletion of USP14 promoted PARP cleavage in the case of cisplatin dosing. Blocking USP14 expression alone did not increase the cleaved PARP level