Figure 3.

EZH2 expression was directly regulated by miR‐144‐3p. A, TargetScan predicted that the binding site of miR‐144‐3p existed at the 3′‐UTR region of EZH2, and dual‐luciferase reporter assay showed that luciferase activity in EZH2‐wt transfected with miR‐144‐3p was lower than that in NC group. **P < 0.01, compared with NC group. B, The expression of EZH2 in tumor tissues was higher than that in adjacent tissues determined by qRT‐PCR. C, D, EZH2 mRNA expression was negatively correlated with miR‐144‐3p mRNA expression in both adjacent tissues and tumor tissues. E, qRT‐PCR analysis indicated that EZH2 mRNA expression in NCI‐H1975 and SPC‐A1 cell lines was much higher than that in BEAS‐2B cell line. F, G, Expression of EZH2 in NCI‐H1975 and SPC‐A1 cells transfected with miR‐144‐3p mimics or si‐EZH2 was lower than that in NC group and the expression after transfected with miR‐144‐3p inhibitor was higher than that in NC group detected by Western blot and qRT‐PCR. **P < 0.01, compared with BEAS‐2B, compared with NC group