FIG 6.

Characterization of mutant strains carrying overexpressed PK variants. (A) Bar chart depicting quantitatively assessed conidiation in the PKOX variants compared with the WT strain, grown on prune agar (PA) medium. Mean values (±SE) were derived from three independent experiments (n = 4 colonies for each sample). Assessments were performed 3 days after photoinduction. n. s., not significant. (B) Disease symptoms on cut leaves of barley inoculated with WT (B157), gcn5Δ mutant, PKOX, and PK(M1)OX, PK(M2)OX conidia in WT and different mutant backgrounds, respectively, were photographed at 7 day postinoculation. (C) Disease symptoms on cut leaves of barley inoculated with WT (B157), PKOX, PK(M1)OX, and PK(M2)OX conidia with or without pyruvate at different concentrations (labeled on top of the images of inoculated leaves), were photographed at 7 days postinoculation. Inoculation with water (H₂O) with 0.25 and 0.50 mM pyruvate on barley leave explants served as a negative control.