FIG 2.

Verification of ribosome footprints. (A) Read mapping analysis of RNA-Seq (gray) and RFPs (purple) on a cluster of three ribosomal protein-coding genes on chromosome 21. Blue arrows delineate annotated coding sequences with gene IDs. Read alignment densities for RNA-Seq (top panel) and RFP (bottom panel) are depicted. (B and C) The 30-nt ribosome footprints (black bars) of the representative sample HSP90rr+RAD were mapped to the annotated CDSs in relation to the initiation (B) and termination (C) sites and plotted by the first nucleotides of the P-sites. Corresponding RNA-Seq reads are shown as blue peaks. Note that peak densities occur at 3-nt intervals for RFPs, representing 3-nt increments of ribosome movement.