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. 2018 Oct 9;7(11):5643–5654. doi: 10.1002/cam4.1807

Figure 3.

Figure 3

CTHRC1 enhances the metastatic ability of cells and promotes the EMT program in CRC cell lines. A, CTHRC1 expression levels were determined by RT‐qPCR in 6 colon cancer cell lines. The data are presented as the mean ± SD (n = 3). B, DLD‐1 cells were transfected with pIRES2‐EGFP‐CTHRC1 or vector for 48 h, and CTHRC1 mRNA levels in these cells were determined by RT‐qPCR with β‐actin as an internal control. The data were compared by a paired t test. C, Ectopic CTHRC1 expression promotes migration and invasion ability in DLD‐1 cells (*P < 0.05). D, Morphological changes after the overexpression of CTHRC1 in DLD‐1 cells. E, The mRNA expression of EMT markers in the CTHRC1 overexpressing DLD‐1 cells determined by RT‐qPCR. The data were compared by a paired t test (*P < 0.05). F, The protein expression of EMT markers in the CTHRC1 overexpressing DLD‐1 cells determined by Western blotting. The data were compared by a paired t test (*P < 0.05)