Figure 3.

DHA inhibits migration and invasion of ovarian cancer cells. An in vitro transwell chamber migration assay and Matrigel invasion assay were used to evaluate the migratory and invasive capabilities of ovarian cancer cells following treatment with 40 μM DHA or DMSO for 24 and 48 h, respectively. A, Images of migrated cells, which were recorded using an Olympus microscope (10×). C, Images of invaded cells, which were recorded using an Olympus microscope (10×). B and D, Average number of migrated and invaded cells from five randomly selected fields. Data represent the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001, compared to controls