Figure 7.

DHA suppresses migration capacity of ovarian cancer through inhibition of the Hh pathway. A, SKOV3 and SKOV3‐IP cells were treated with purmorphamine (1.5 µM) alone, DHA (40 µM) alone, or a combination of DHA and purmorphamine for 24 h. C, SKOV3 and SKOV3‐IP cells were treated with GANT61 (40 µM), DHA (40 µM), or a combination of DHA and GANT61 for 24 h. A and C, Images of migrated cells, which were recorded using an Olympus microscope (10×). B and D, Average number of migrated cells from five randomly selected fields represented by the histogram. Data are represented as the mean ± SEM of three separate experiments. *P < 0.05, **P < 0.01 and ***P < 0.001, compared with controls. ^# P < 0.05, ^## P < 0.01 and ^### P < 0.001, vs the purmorphamine and GANT61 groups