FIG 1.

AgNO₃ growth inhibition of R. gelatinosus. Wild-type cells were grown in 96-well microplates in the Tecan Infinite M200 luminometer. Indicated concentrations of CuSO₄, CdCl₂, or AgNO₃ were added to the growth medium after 3.5 h, when cells reached an OD₆₈₀ of 0.16 (arrow). Black line represents growth curves of untreated cells (malate) and red, green, blue, cyan, magenta, yellow, and brown lines represent growth curves of cells treated with increasing concentrations of Cu²⁺, Cd²⁺, or Ag⁺, as indicated.