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. 2018 Nov 20;9(6):e01535-18. doi: 10.1128/mBio.01535-18

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Copyright © 2018 Tambosi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 8 — AgNO₃ effect on respiratory complexes in E. coli and B. subtilis cells. Strains were grown under aerobic respiratory condition and shocked for 1h with increasing concentration of AgNO₃. DDM-solubilized membrane proteins were separated on a 5% to 12% gradient BN-PAGE. (A) Succinate dehydrogenase (SDH) in-gel activity assay of E. coli membrane fractions. Membrane fraction from the SDH-deficient mutant (sdhA⁻) was used as a control. (B) caa₃ cytochrome c oxidase and succinate dehydrogenase (SDH) in-gel activity assays of B. subtilis membranes.