FIG 5.

Disruption of C. neoformans Cda1 catalytic site did not interfere with its cellular localization. The indicated strains were streaked onto YPD agar and incubated for 4 days at 30°C. Cells were collected from the plates, washed with PBS, and subjected to subcellular fractionation. Equal quantities of protein as determined by the BCA method were separated on 10% SDS gel and analyzed by Western blotting using a C. neoformans Cda1-specific polyclonal antibody. Lanes: L, total cell lysate; C, cytosol fraction; M, cell membrane fraction; CW, cell wall fraction.