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. 2018 Jul 10;6(4):416–427. doi: 10.1002/iid3.226

Figure 1.

Figure 1

Expression of cell surface receptors on resting and activated PBdMCs from non‐allergic (n = 8–10) and peanut allergic (n = 7–8) participants. PBdMC were harvested and incubated at 37°C overnight with medium (resting cells) or medium containing human IgE to allow the cells to become sensitized. PBdMC were washed and incubated at 37°C for 18 h with (Stimulated) or without anti‐IgE (Sensitized). PBdMC were then stained with anti‐FcϵRI‐PE‐Cy7 (AER‐37(CRA‐1)), anti‐CD117‐BV650 (104D2), anti‐CD203C‐BV510 (NP4D6), anti‐C3aR‐AF647 (hC3aRZ8), anti‐CD88‐PE‐Cy5 (S5/1), anti‐CD48‐BV421 (TÜ145), anti‐Siglec‐8‐AF700 (837535), anti‐CD200R‐PE (OX‐108), anti‐CD300a‐AF647 (MEM‐260) and anti‐CD32‐FITC (FLI8.26). Statistics were performed using student t‐test with Bonferroni correction for multiple comparisons. Comparing non‐allergic with peanut allergic within each condition (resting, sensitized, and stimulated) did not reach statistical significance for any of the receptors. Gray bars = non‐allergic, Black bars = peanut allergic.