Figure 4. Effects of Astragalus polysaccharides (APS) on MG63 cells were reversed by miR-133a inhibition. A, After miR-133a inhibitor transfection, the expression of miR-133a in MG63 cells was determined by quantitative reverse transcription PCR. Transfected and non-transfected cells were stimulated with 10 mg/mL APS. Non-treated cells acted as control (NC). B, Percentage of BrdU positive cells was detected by BrdU incorporation assay. C, Expression levels of proliferation-associated proteins were assessed by western blot analysis. D, Percentage of apoptotic cells was measured by flow cytometry. E, Expression levels of apoptosis-associated proteins were evaluated by western blot analysis. F, Cell migration was determined by transwell migration assay. G, Expression levels of migration-associated proteins were detected by western blot analysis. MMP: matrix metalloproteinase. H, Cell invasion was measured by transwell invasion assay. I, Expression of invasion-associated protein was evaluated by western blot analysis. Data are reported as means±SE of three independent experiments. *P<0.05; **P<0.01 (ANOVA).