Figure 6. Oridonin induced DNA damage in SNU-216 cells. SNU-216 cells were transfected with si-p53 to silence p53 expression. Then, transfected cells or non-transfected cells were treated with oridonin (40 μM) for 0 h, 3 h, and 6 h. The protein expression of p53 and γ-H2AX was measured by western blot. A, Blots of p53 and γ-H2AX in non-transfected cells. B, Blots of p53 and γ-H2AX in transfected cells. C, Quantification of relative signal intensity of blots in non-transfected cells and transfected cells. NC, negative control of si-p53. Data are reported as means±SD. *P<0.05, **P<0.01, ***P<0.001 compared to 0 h (ANOVA).