Figure 7. Role of p53 and caspase in the anti-tumor effect of oridonin. SNU-216 cells were transfected with si-p53 #1 or si-p53 #2. A, Transfected efficiency was verified by western blot. SNU-216 cells were transfected with si-p53 #1 or si-p53 #2, and then the transfected cells were exposed to oridonin (40 μM) for 24 h. B, Cell viability was measured by CCK-8 assay. C, Expression of p53, cleaved-caspase-3, and cleaved-caspase-9 was determined by western blot. SNU-216 cells were incubated with oridonin (40 μM) or combined with caspase inhibitor, Boc-D-FMK. D, Cell viability and E, expression of cleaved-caspase-3 and cleaved-caspase-9 were measured by CCK-8 assay and western blot, respectively. NC, negative control of si-p53 #1 and si-p53 #2. Data are reported as means±SD. *P<0.05, **P<0.01, ***P<0.001 (ANOVA).
