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. 2018 Nov 20;131(22):2726–2733. doi: 10.4103/0366-6999.245271

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Copyright: © 2018 Chinese Medical Journal

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EMP^hypoxia inhibited migration and angiogenesis of HUVECs. HUVECs treated with EMP^control and EMP^hypoxia were subjected to scratch migration, transwell migration, and tube formation assay. (a) Scratch migration image. Migration analysis was measured as a percentage of total cell-free area. (b) Transwell migration image. Migration analysis was measured as the total migration cell number. (c) Representative images of tube formation and the relative number of tube branches measured in random 10 photographic fields were presented. *P < 0.001, ^†P < 0.01 compared with EMP^control group. EMP: Endothelial microparticle; HUVECs: Human umbilical vein endothelial cells.