Fig. 9.

Dexamethasone induces Muc1-CT nuclear translocation and co-localization with GRα in bronchial epithelium of WT mice but not in MUC1 KO mice. C57BL/6 Muc1 KO mice and WT mice were undergoing dexamethasone 10 mg/kg/day (orally) for 6 days. Animals were sacrificed at day 6 and lungs were fixed in paraformaldehyde (4%) for 48 h and embedded in Tissue-Tek® OCT™ cryosectioning compound. Lung slices were immunostained with MUC1-cytoplasmic tail (CT) and glucocorticoid receptor alpha (GRα) antibodies with rhodamine/fluorescein secondary antibodies. DAPI was used to mark cell nucleus. Representative con-focal images are showed. Scale bar: 5 μm