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Specificity of RT-PCR and real-time RT-PCR assay. PDCoV and negative samples were detected by RT-PCR and RT-qPCR. Negative samples included SADS-CoV, PRRSV, PRV, CSFV, PCV2, TGEV, PEDV, RV, PPV, FMDV, SIV, and distilled water. (a) Only PDCoV genome was amplified in RT-PCR. (b) PDCoV was found to have a strong fluorescence signal under RT-qPCR; the Ct values of negative samples were greater than 35. (c) Melting peak was observed at 86°C for only PDCoV in RT-qPCR.
