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Primers used in this study.
| Name | Sequence |
|---|---|
| N-F | ACGCTGCTGATTCCTGCT |
| N-R | GCTACTCATCCTCAGTTTCGTG |
| PCR-F | TGGAACTGACCCGGATGTTG |
| PCR-R | GCGTACACCCTAGTGGCTTC |
| qPCR-F | TTCCTATGGAGATGACCTATTAATTGGAAC |
| qPCR-R | TCAGAGGAAAGGTGGTGGTCTTGTTGGCAG |
The primer N-F/R was used for the amplification of whole n gene. The primer PCR-F/R was used for performing conventional RT-PCR. The primer qPCR-F/R was used for performing RT-qPCR.
