Table I:
Extracellular superoxide (O2−) and hydrogen peroxide (H2O2) production by the organisms examined in this study
| Species | Strain | Dilution level | cells mL−1 | ROS Production | |
|---|---|---|---|---|---|
| fmol cell−1 h−1 | |||||
| O2− | H2O2 | ||||
| Chattonella marina | ARC260 | 1:1 | 14 000 | 49 ± 1 (n = 4) | 87 ± 3 (n = 4) |
| 1:10 | 1400 | 40 ± 11 (n = 4) | 162 ± 35 (n = 3) | ||
| 1:100 | 140 | 68 ± 24 (n = 2) | 2658 ± 408 (n = 4) | ||
| Filtrate | 0 | 5 ± 1 (n = 4) | 12 ± 1 (n = 4) | ||
| Heterosigma akashiwo | ARC114 | 1:1 | 150 000 | 0.9 ± 0.3 (n = 4) | BD |
| 1:10 | 15 000 | 10 ± 2 (n = 4) | BD | ||
| 1:100 | 1500 | 95 ± 15 (n = 3) | BD | ||
| Filtrate | 0 | 1.5 ± 0.2 (n = 4) | 1.2 ± 0.1 (n = 4) | ||
| Karenia brevis | ARC5 | 1:1 | 5300 | 12 ± 2 (n = 4) | 5 ± 1 (n = 4) |
| 1:10 | 530 | 50 ± 18 (n = 4) | 19 ± 6 (n = 3) | ||
| 1:100 | 53 | BD | 397 ± 45 (n = 3) | ||
| Filtrate | 0 | BD | BD | ||
| Pseudo-nitzschia sp. | ARC447 | 1:1 | 10 000 | 10 ± 9 (n = 2) | 0.9 ± 0.2 (n = 4) |
| 1:10 | 1000 | 118 ± 35 (n = 4) | 4 ± 1 (n = 3) | ||
| 1:100 | 100 | 431 ± 248 (n = 3) | 56 ± 21 (n = 4) | ||
| Filtrate | 0 | 24 ± 2 (n = 4) | BD | ||
| Aureococcus anophagefferens | CCMP1984 | 1:1 | 210 000 | 0.1 ± 0.1 (n = 4) | BD |
| 1:10 | 21 000 | 0.6 ± 0.2 (n = 4) | 0.7 ± 0.1 (n = 4) | ||
| 1:100 | 2100 | 10 ± 3 (n = 3) | 8 ± 1 (n = 4) | ||
| Filtrate | 0 | 0.1 ± 0.0 (n = 4) | 0.1 ± 0.0 (n = 4) | ||
The ROS production rates in cell-free filtrates were normalized to the original cell density in the undiluted (1:1) culture at the time of sampling. Mean ROS production rates ± standard error of the mean are provided. BD, below detection.