Table 3.

Main mutations obtained by PoPMuSiC program.

	Hot points	Shift	ΔΔG (kcal/mol)
1	203	PRO → VAL	−1.91
2	203	PRO → CYS	−1.89
3	487	GLU → ILE	−1.75
5	487	GLU → TYR	−1.75
6	170	ASP → TYR	−1.5
7	170	ASP → PHE	−1.47
8	399	ASP → MET	−1.37
9	142	GLY → PHE	−1.35
10	183	PRO → TRP	−1.2

The 10 mutants (shift residue) with the best and favorable energy in contrast to Lacc 6. The amino acid sequence number is found in second colum, in the next columna is the hot poin residue and their shift, the last column presnt the ΔΔG between Lacc 6 and mutant protein.