Figure 1.

Detection of reporter gene expression in recombinant adeno-associated virus (rAAV)-transduced human bone-marrow aspirates seeded in three-dimensional (3D)-woven poly(ɛ-caprolactone; PCL) scaffolds. Aspirates (150 mL) were transduced with rAAV-lacZ or rAAV-RFP (100 mL each vector) or left untreated and seeded in the scaffolds in fibrinogen/thrombin using chondrogenic medium, as described in the Methods. The samples were processed at the denoted time points to detect (A) lacZ expression by X-Gal staining (macroscopic views and magnification 40 × ; all representative data) and (B) RFP expression by live fluorescence (inserts: rAAV-lacZ control treatment; magnification 40 × and macroscopic views at day 75; all representative data). Scale bars: (A) 3 mm (scaffold), 1 cm (aspirates), and 100 mm (histological sections); (B) 200 mm. Color images available online at www.liebertpub.com/hum