Figure 3.

Chondrogenic differentiation in rAAV-transduced human bone-marrow aspirates seeded in 3D-woven PCL scaffolds. Aspirates were transduced with rAAV-lacZ or rAAV-FLAG-hsox9 or left untreated and seeded in the scaffolds in fibrinogen/thrombin using chondrogenic medium, as described in Figs. 1 and 2 and in the Methods. The samples were processed after 21 days for histological staining with safranin O and hematoxylin and eosin and to detect immunoreactivity to type II collagen (magnification 10 × ; all representative data). Scale bars: 200 mm. H&E, hematoxylin and eosin. Color images available online at www.liebertpub.com/hum