Figure 4.

Hypertrophic and terminal differentiation in rAAV-transduced human bone-marrow aspirates seeded in 3D-woven PCL scaffolds. Aspirates were transduced with rAAV-lacZ or rAAV-FLAG-hsox9 or left untreated and seeded in the scaffolds in fibrinogen/thrombin using chondrogenic medium, as described in Figs. 1–3 and in the Methods. The samples were processed after 21 days for histological staining with alizarin red and to detect immunoreactivity to type I and X collagen (magnification 10 × ; all representative data). Scale bars: 200 mm. Color images available online at www.liebertpub.com/hum