Figure 5.

Real-time reverse transcription polymerase chain reaction analysis in rAAV-transduced human bone-marrow aspirates seeded in 3D-woven PCL scaffolds. Aspirates were transduced with rAAV-lacZ or rAAV-FLAG-hsox9 or left untreated and seeded in the scaffolds in fibrinogen/thrombin using chondrogenic medium, as described in Figs. 1–4 and in the Methods. The samples were processed after 21 days to monitor the gene expression profiles of SOX9, aggrecan (ACAN), type-II (COL2A1), type-I (COL1A1), and type-X collagen (COL10A1), with GAPDH serving as a housekeeping gene and internal control (all primers are listed in the Methods). Ct values were obtained for each target and for GAPDH as a control for normalization, and fold inductions (relative to the untreated samples) were measured by using the 2^–ΔΔCt method. Statistically significant compared with *no vector treatment and with ⁺rAAV-lacZ.