Table 1.
Histomorphometric analyses in rAAV-transduced human bone-marrow aspirates seeded in 3D-woven PCL scaffolds
| Parameter | No vector | lacZ | SOX9 |
|---|---|---|---|
| SOX9 | 0.5 (0.4) | 0.5 (0.6) | 3.5 (0.6)*,† |
| Safranin O | 1.8 (0.3) | 1.9 (0.4) | 3.4 (0.2)*,† |
| Type-II collagen | 1.5 (0.6) | 1.5 (0.5) | 3.8 (0.4)*,† |
| Cell densities | 2358 (56) | 2422 (39) | 2463 (44) |
| Alizarin red | 3.6 (0.4) | 3.4 (0.5) | 1.3 (0.3)*,† |
| Type I collagen | 2.8 (0.5) | 2.8 (0.4) | 1.1 (0.4)*,† |
| Type X collagen | 3.6 (0.7) | 3.4 (0.4) | 1.1 (0.1)*,† |
SOX9 immunostaining, safranin O staining, type-II collagen immunostaining, alizarin red staining, and types I and X collagen immunostaining were scored for uniformity and intensity as: 0, no staining; 1, heterogeneous and/or weak staining; 2, homogeneous and/or moderate staining; 3, homogeneous and/or intense staining; and 4, very intense staining.46 The cell densities measured on H&E-stained histological sections are given in cells/mm2. Values are given as means (SD; n = 3). Statistically significant compared with *no vector treatment and with †rAAV-lacZ.
rAAV, recombinant adeno-associated virus; 3D, three-dimensional; PCL, poly(ɛ-caprolactone); H&E, hematoxylin and eosin; SD, standard deviation.